The addition of Andean lupin (Lupinus mutabilis) protein concentrate enhances the nutritive value and the antioxidant activity of yoghurt
Yoghurts added with Andean lupin protein concentrate
DOI:
https://doi.org/10.14306/renhyd.26.S1.1406%20Keywords:
lupino; proteínas; ácidos grasos insaturados; polifenoles; flavonoides; índice aterogénico; enfermedad cardiovascularAbstract
Introduction: Andean lupin seeds are under-exploited sources of nutrients and bioactive compounds.
Materials and methods: seeds were debittered to remove toxic alkaloids, partially defatted with 70% ethanol, dried at 60°C and milled. The defatted flour was suspended in water adjusted at pH 7 with 1N NaOH. The protein extract was filtered and spray-dried. Enriched yoghurts were obtained with the addition of lupin protein concentrate at 0.5, 1 and 1.5%. The chemical and fatty acid composition, the contents of polyphenols and flavonoids as well as the antioxidant activity were determined. The microbial quality and physicochemical properties (pH values, lactic acid content and percentage of syneresis) were evaluated in yoghurts during 28 days of storage.
Results: Andean lupin concentrate showed high protein and carbohydrate contents (69% and 20%), unsaturated fatty acids (77%) mainly oleic, linoleic and α-linolenic acids as well as low atherogenic and thrombogenic indexes (0.07 and 0.44). The addition of the lupin protein concentrate at 1.5% into yoghurts increased the protein content. Enriched yoghurts showed higher unsaturated fatty acids, total polyphenol and flavonoid contents as well as lower atherogenic and thrombogenic indexes compared to the control. The lupin protein concentrate increased the ABTS radical scavenging activity, the ferric reducing power and the total antioxidant activity in yoghurts. Enriched and control yoghurts showed similar lactic acid bacterial counts. The counts for total and thermotolerant coliforms were under three MPN/mL and yeast and molds under 10 CFU/ mL. The pH values in enriched products were higher at days 1 and 7 of storage (4.82 and 4.95) compared to the control sample (4.72 and 4.85). The lactic acid content increased in all formulations at day 21. Enriched yoghurts showed lower syneresis than the control.
Conclusions: Yoghurts enriched with lupin protein concentrate could represent nutritive and functional alternatives to prevent chronic diseases when consuming in a healthy diet.
References
(1) Carvajal-Larenas F, Linnemann A, Nout M, Koziol M, van Boekel MAJS. Lupinus mutabilis : composition , uses , toxicology and debittering. Crit Rev Food Sci Nutr. (2016); 84: 37–41.
(2) Campos D, Chirinos R, Gálvez L, Pedreschi R. Bioactive potential of Andean fruits, seeds, and tubers. Adv Food Nutr Res (2018); 84: 287-343.
(3) Gálvez-Ranilla L, Genovese MI, Lajolo FM. Isoflavones and antioxidant capacity of Peruvian and Brazilian lupin cultivars. J Food Compost Anal (2009); 22: 397–404.
(4) Australia New Zealand Food Authority. Lupin alkaloids in food. A Toxicological Review and Risk Assessment. The Authority Canberra (2001).
(5) Carvajal-Larenas F, Nout M, van Boekel MAJS, Koziol M, Linnemann AR. Modelling of the aqueous debittering process of Lupinus mutabilis Sweet. LWT - Food Sci Technol. (2013); 53: 507–516.
(6) Carvajal-Larenas F. Nutritional, rheological and sensory evaluationof Lupinus mutabilis food products - a review. Czech J Food Sci. (2019); 37: 301–311
(7) Schindler S, Wittig M, Zelena K, Kringsa U, Bez J, Eisner P, Bergera, RG. Lactic fermentation to improve the aroma of protein extracts of sweet lupin (Lupinus angustifolius). Food Chem. (2011); 128: 330–337
(8) Fazilah N, Ariff A, Khayat M, Rios-Solis L, Halim M. Influence of probiotics, prebiotics, synbiotics and bioactive phytochemicals on the formulation of functional yogurt. J Funct Foods. (2018); 48: 387–399.
(9) Agil R, Gaget A, Gliwa J., Avis TJ, Willmore WG, Hosseinian F. Lentils enhance probiotic growth in yogurt and provide added benefit of antioxidant protection. LWT- Food Sci Technol. (2013); 50: 45–49.
(10) Chen X, Singh M, Bhargava K, Ramanathan R. Yogurt fortification with chickpea (Cicer arietinum) flour: physicochemical and sensory effects. JAOCS. (2018); 95: 1041–1048.
(11) Zare F, Champagne C, Simpson B, Orsata V, Boye JI. Effect of the addition of pulse ingredients to milk on acid production by probiotic and yoghurt starter cultures. LWT - Food Sci Technol. (2012); 45; 155–160.
(12) Instituto Ecuatoriano de Nomarlización. Legumbres. Grano desamargado de chocho. Gobierno de Ecuador (2005).
(13) Kwiatkowski JR, Cheryan M. Extraction of oil from ground corn using ethanol. JAOCS. (2002); 79: 825–830.
(14) Ministério da Agricultura Pecuaria e Abastecimento. Regulamento Técnico de identidade e qualidade de leites fermentados. Diário Oficial da União. (2007); 16.
(15) Association of Official Analytical Chemists – AOAC. Official methods of analysis of the Association of Official Analytical Chemists (16th ed.). Arlington: Association of Analytical Communities. 1995
(16) Association of Official Analytical Chemists – AOAC. Official methods of analysis of the Association of Official Analytical Chemists. Fat in milk. (13th ed.). Washington: Association of Analytical Communities. 1992
(17) Association of Official Analytical Chemists – AOAC. Official methods of analysis of the Association of Official Analytical Chemists. Fat (Total, Saturated, and Unsaturated) in Foods (16th ed.). Arlington: Association of Analytical Communities. 2001.
(18) Ulbritch T, Southgate D. Coronary heart disease: sev-en dietary factors. The Lancet (1991); 338: 985–992
(19) Nguyen L, Hwang ES. Quality characteristics and antioxidant activity of yogurt supplemented with aronia (Aronia melanocarpa) juice. Prev Nutr Food Sci (2016); 21: 330–337.
(20) Raikos V, Ni H, Hayes H, Ranawana V. Antioxidant Properties of a Yogurt Beverage Enriched with Salal (Gaultheria shallon) Berries and Blackcurrant (Ribes nigrum) Pomace during Cold Storage. Beverages. (2018); 5: 1-11.
(21) Singleton V, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol. (1998); 299: 152-178.
(22) Re R, Pellegrini N, Proteggente A, Pannla A. Antioxidant Activity Applying an Improved ABTS Radical cation decoloration assay. Free Radic. Biol. Med. (1999); 26: 1231–1237
(23) Umamaheswari M, Chatterjee T. In vitro antioxidant activities of the fractions of Coccinia grandis L. leaf extract. AJTCAM. (2007); 27: 61–73.
(24) Valdés-Izaguirre L, Arias-Cedeño Q, Ramírez-Arzuaga J, Peña-Fuentes D (2018). Actividad antiinflamatoria y antioxidante in vitro de extractos etanólicos de Jatropha aethiopica Müell Arg var inermis. Rev. Cubana Quím. (2018); 30 (3): 440-453.
(25) American Public Health Association - APHA. Compendium of Methods for the Microbiological Examination of Foods. 2015.
(26) Association of Official Analytical Chemists – AOAC. Official methods of analysis of the Association of Official Analytical Chemists. Acidity of milk. Titrimetric method. Association of Analytical Communities. 1947.
(27) Antunes AE, Antunes AJ, Cardello, HM. Chemical, physical, microstructural and sensory properties of set fat-free yogurts stabilized with whey protein concentrate. Milchwissenschat. (2004); 59: 161-165.
(28) Di renzo JA, Casanoves F, Balzarini MG. InfoStat software. Student Version. Córdoba, Argentina. https://www.infostat.com.ar
(29) Villacrés E, Quelal MB, Jácome X, Cueva G, Rosell C. Effect of debittering and solid-state fermentation processes on the nutritional content of 10 lupine (Lupinus mutabilis Sweet). Int. J. Food Sci. Technol (2020); 55: 2589-2598.
(30) Xua Y, Codaa R, Holopainen-Mantila U, Laitila A, Katina K, Tenkanen M. Impact of in situ produced exopolysaccharides on rheology and texture of fava bean protein concentrate. Food Res. Int.. (2019); 115: 191-199.
(31) Cheftel JC, Cheftel H. Introduction a la Biochimie et a la Technologie des Aliments. Technique et documentation 243-330. 1992 Paris
(32) Bader S, Palomino Oviedo J, Pickardt C, Eisner P. Influence of different organic solvents on the functional and sensory properties of lupin (Lupinus angustifolius L.) proteins. LWT - Food Sci Technol. (2011); 44: 1396-1404.
(33) Calabrò S, Cutrignelli MI, Lo Presti V, Tudisco R, Chiofalo V, Grossi M, Infascelli F, Chiofalo B. Characterization and effect of year of harvest on the nutritional properties of three varieties of white lupine (Lupinus albus L.). J. Sci. Food Agric. (2015); 95: 3127–3136.
(34) Chen J, Liu H. Nutritional Indices for Assessing Fatty Acids: A Mini-Review. Int. J. Mol. Sci. (2020); 21, 5695.
(35) Siger A, Czubinski J, Kachlicki P, Krzyszto P, Dwieckia K, Lampart Szczapa E, Nogala-Kalucka M. Antioxidant activity and phenolic content in three lupin species. Food Compost Anal. (2012); 25: 190-197.
(36) Sreerama YM, Sashikala, VB, Pratape V. Phenolic compounds in cowpea and horse gram flours in comparison to chickpea flour: Evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia and hypertension. Food Chem. (2012); 133: 156-162.
(37) Haq F, Sameen A, Zaman Q, Mushtaq BS, Hussain M, Javed A, Plygun S, Korneeva O, Shariati MA. Development and evalutation of yogurt supplemented with lentil flour. J. Microbiol. Biotechnol. Food Sci. (2018); 8 (4): 1005-1009.
(38) Pahariya P. Effects of yogurt fortification with different legumes protein on the physio-Chemical, microbiological, and rheological properties. Thesis of Master of Sciences. South Dakota, United States. Open public research access institutional repository and information exchange. (2018).
(39) Gamli OF, Atasoy AF. Physico-chemical and sensorial properties of groundnut milk and it’s yoghurt. J. Food Meas. Charact. (2018); 12: 1997–2004.
(40) Bayat AR, Tapio I,Vilkki J, Shingfield KJ, Leskinen H. Plant oil supplements reduce methane emissions and improve milk fatty acid composition in dairy cows fed grass silage-based diets without affecting milk yield. J. Dairy Sci. (2018); 101: 1136-1151.
(41) Boeck T, Sahin AY, Zannini E, Arendt EK. Nutritional properties and health aspects of pulses and their use in plant-based yogurt alternatives. Compr. Rev. Food Sci. Food Saf. (2021); 20: 3858-3880.
(42) Lee W, Lucey J. Formation and physical properties of yoghurt. Asian-australas J Anim Sci. (2010); 23: 1127–1136.
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